L10066  Ichihashi New Phalaenopsis Medium (NP-medium)

Instant medium as described by S. Ichihashi 1992 for micropropagation of Phalaenopsis
through culture of lateral young flower buds (Lindleayana 7(4): 208-215).
For sowing and tissue culture of Phalaenopsis hybrids and other vandaceous orchids.
Does not contain plant growth regulators (PGR)! For tissue culture purposes PGR
should be added according to the desired effect. Ichihashi recommends to adjust the
pH to 5.1. An optional ingredient is coconut water at 150 ml/l, but Ishihashi observed
both enhancing and inhibitory effects, thus this product does not contain coconut.
26.2892 g per liter.
Hygroscopic! Sealed in heavy duty plastic bag. To be
used immediately after opening.
Sterilization: autoclavable
Storage Temp.: RT (max. 1 year). For longer shelf life (>1 year) store at 4 ˇăC, for maximum
shelf life (>3 years) store at -20 ˇăC or below. Use up opened bags immediately and
do not store left-over powder from open bags!

Medium composition:
KNO3 0.4246 g/L
NH4NO3 0.0320 g/L
(NH4)2SO4 0.3039 g/L
Ca(NO3)2 ˇ¤ 4H2O 0.6376 g/L
KH2PO4 0.4627 g/L
Mg(NO3)2 ˇ¤ 6H2O 0.2564 g/L
Na2EDTA (Prod. # L20167) 0.0373 g/L
FeSO4 x 7 H2O 0.0278 g/L
Ichihashi trace elements (Prod. # L30008) 0.0038 g/L
MS orig. Vitamins (Prod. # L20081) 0.1031 g/L
Sucrose 20.0000 g/L
Gellanum Gum (Hengdugel, Prod. # L20111) 4.0000 g/L

 

Close