L10050  Murashige & Skoog Orchid-medium 1a (MSO 1a)

Instant medium with complex additives and activated charcoal.
Complete with sugar but no agar! Does not contain plant growth regulators.
For sowing or tissue culture of a wide range of tropical orchids. Note that
the activated charcoal absorbs a considerable amount of plant growth regulators
(PGR) if these are added to the medium. For better performance in tissue culture
with PGR please use MSO 1b, Prod. # 10051. If further ingredients are added
to this product the pH needs to be re-adjusted!
27.4075 g per liter.
Hygroscopic! Sealed in heavy duty plastic bag. To be
used immediately after opening.
Sterilization: autoclavable

Medium composition:
KNO3 0.9500 g/L
NH4NO3 0.8250 g/L
KH2PO4 0.0850 g/L
MgSO4 กค 7H2O 0.1850 g/L
CaCl2 anhydr. 0.1660 g/L
Na2EDTA (Prod. # L20167) 0.0372 g/L
FeSO4 x 7 H2O 0.0279 g/L
MS mod. trace elements (Prod. # L30002) 0.0174 g/L
MS mod. Vitamins (Prod. # L20082) 0.1140 g/L
MES (Prod. # L20170) 1.0000 g/L
Peptone (Prod. # L20121) 2.0000 g/L
Activated charcoal (Prod. # L20161) 2.0000 g/L
Sucrose 20.0000 g/L
pH pre-adjusted with KOH

 

Close