L10033  Knudson C, Anderson modification

Complete medium with potato, vitamins, glucose and agar as
published by Allan B. Anderson in 1990. Modified Knudson C
with slightly reduced salt contents, improved iron delivery and
slight buffer capacity. Anderson used it for successfully sowing
Spiranthes cernua. Also useful for tropical orchids.
25.6581 g per liter.
Hygroscopic! Sealed in heavy duty plastic bag. To be
used immediately after opening.
Sterilization: autoclavable
Storage Temp.: RT (max. 1 year). For longer shelf life (>1 year) store at 4 ˇăC, for maximum
shelf life (>3 years) store at -20 ˇăC or below. Use up opened bags immediately and
do not store left-over powder from open bags!

Medium composition:
(NH4)2SO4 0.2500 g/L
Ca(NO3)2 x 4 H2O 1.0000 g/L
KH2PO4 0.2280 g/L
K2HPO4 0.0078 g/L
MgSO4 ˇ¤ 7H2O 0.1250 g/L
(NH4)2-Fe(III)-Citrate (28 % Fe) 0.0250 g/L
Thiamine-HCl (Vit. B1) 0.0050 g/L
Nicotinic Acid (Vit. B3, Niacin) 0.0100 g/L
Ca-Pantheonate (Vit. B5) 0.0050 g/L
Pyridoxin (Vit. B6) 0.0020 g/L
Biotin 0.00025 g/L
Potato powder (Prod. # L20128) 2.0000 g/L
Glucose 16.0000 g/L
Hengduagar (Prod. # L20112) 6.0000 g/L
pH pre-adjusted with KOH

 

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