L10034  Knudson C, OSSSU-modification (KCO)

Complete instant medium with sugar and agar, without PGR.
For sowing a wide range of tropical orchids.
This medium was published by Joseph Arditti et al. in 1982 and is the adapted standard
reference medium for sowing orchid seeds out of storage in the international conservation
project 'Orchid Seed Storage for Sustainable Use' (OSSSU), in which Hengduan Mts.
Biotechnology is taking part in. KCO is a slight modifcation of the original Knudson C. Our
Product has a pre-adjusted pH and is ready for immediate use.
28.5 g per liter.
Hygroscopic! Sealed in heavy duty plastic bag. To be
used immediately after opening.
Sterilization: autoclavable
Storage Temp.: RT (max. 1 year). For longer shelf life (>1 year) store at 4 <C, for maximum
shelf life (>3 years) store at -20 <C or below. Use up opened bags immediately and
do not store left-over powder from open bags!

Medium composition:
Ca(NO3)2 , 4H2O 1.0000 g/L
(NH4)2SO4 0.5000 g/L
KH2PO4 0.2500 g/L
MgSO4 , 7H2O 0.2500 g/L
FeSO4 , 7H2O 0.0250 g/L
MnSO4 , H2O 0.0057 g/L
H3BO3 0.000056 g/L
CuSO4 , 5H2O 0.0000624 g/L
MoO3 0.000016 g/L
ZnSO4 , 7H2O 0.000331 g/L
KOH 0.0500 g/L
Sucrose 20.0000 g/L
Hengduagar (Prod. # L20112) 6.4188 g/L
pH pre-adjusted with KOH to 5.6 - 5.8

 

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