L20089  Gamborg B5 Vitamins

This is the original mix of vitamins and myo-inositol as published
by O.L. Gamborg, R.A. Miller and K. Ojima 1968.
to filter sterilize the vitamins and glycine and add them to the
medium after autoclaving. To prepare a 10% stock solution
dissolve 1.027 g in 10 ml hot water or  10.27 g in 100 ml hot water.
1 ml of this stock solution has to be added per liter medium.
Remaining stock solution can be stored for a few weeks in a
refrigerator, but myo-inositol might precipitate and the crystals
have to be re-dissolved by heating up the stock solution.
Mind water loss during heating!
This vitamin mix is provided as a powder and should be used
in powder form to insure maximum shelf life. This product is only
for use in plant tissue culture!
0.112 g per liter.
Powder, packed in a light proof and airtight PP flask. Store in a
cold and dry place, keep container always tighly closed.
Sterilization: autoclavable, partly decomposing!
Storage Temp.: 4 ˇăC

 

Product composition:
Thiamin ˇ¤ HCl 10.00 mg/L
Nicotinic acid (Niacin) 1.00 mg/l
Pyridoxin ˇ¤ HCl 1.00 mg/L
myo-Inositol 100.00 mg/L
(all ingredients USP grade or higher)

 

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