This is the original mix of vitamins and myo-inositol as published |
by O.L. Gamborg, R.A. Miller and K. Ojima 1968. |
to filter sterilize the vitamins and glycine and add them to the |
medium after autoclaving. To prepare a 10% stock solution |
dissolve 1.027 g in 10 ml hot water or 10.27 g in 100 ml hot water. |
1 ml of this stock solution has to be added per liter medium. |
Remaining stock solution can be stored for a few weeks in a |
refrigerator, but myo-inositol might precipitate and the crystals |
have to be re-dissolved by heating up the stock solution. |
Mind water loss during heating! |
|
This vitamin mix is provided as a powder and should be used |
in powder form to insure maximum shelf life. This product is only |
for use in plant tissue culture! |
|
0.112 g per liter. |
Powder, packed in a light proof and airtight PP flask. Store in a |
cold and dry place, keep container always tighly closed. |
Sterilization: autoclavable, partly decomposing! |
Storage Temp.: 4 ˇăC |