L10069  Fonnesbech Cymbidium TC medium

Instant liquid medium as developed by M. Fonnesbech 1972 for tissue
culture of Cymbidium hybrids (Physiol. Plant. 27, pages 310-312 and
and 360-364). The composition is as cited in J. Arditti and R. Ernst,
1993 (Micropropagation of Orchids). The medium was designed for
proliferation after a 3-stage establishing culture on modified Knudson C,
with the third stage containing 150 g/L banana pulp.
For optimal performance 100-150 ml coconut water have to be added.
Due to its granular structure coconut powder has not been added to
this liquid medium instant powder!
34.1995 g per liter.
Hygroscopic! Sealed in heavy duty plastic bag. To be
used immediately after opening.
Sterilization: autoclavable
Storage Temp.: RT (max. 1 year). For longer shelf life (>1 year) store at 4 ˇăC, for maximum
shelf life (>3 years) store at -20 ˇăC or below. Use up opened bags immediately and
do not store left-over powder from open bags!

Medium composition:
(NH4)2SO4 0.3000 g/L
Ca(NO3)2 ˇ¤ 4H2O 0.4000 g/L
KH2PO4 0.2500 g/L
K2HPO5 0.2120 g/L
MgSO4 ˇ¤ 7H2O 0.2500 g/L
Na2EDTA (Prod. # L20167) 0.0378 g/L
FeSO4 x 7 H2O 0.0279 g/L
MnSO4 x H2O 0.00655 g/L
H3BO3 0.0062 g/L
CuSO4 x 5H2O 0.000025 g/L
Co(NO3)2 x 6H2O 0.000025 g/L
Na2MoO4 x 2H2O 0.00025 g/L
KI 0.00075 g/L
ZnSO4 x 7H2O 0.00267 g/L
Thiamine x HCl 0.0001 g/L
Nicotinic acid 0.0005 g/L
Pyridoxine x HCl 0.0005 g/L
Glycine 0.0020 g/L
myo-Inositol 0.1000 g/L
NAA (1-Naphtalenacetic acid) 0.00186 g/L
Kinetin 0.000215 g/L
Caseinhydrolysate 2.5000 g/L
Sucrose 30.0000 g/L

 

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