L10117  Svante Malmgren Medium 1b (SMM 1b)

Complete instant medium with gelling agent, ready to use.
This is a revised composition by Svante Malmgren as published
by Raennbaeck in 2007 for sowing terrestrial European orchids.
It contains amino acids and vitamins as described by Malmgren.
Malmgren uses NH3 in water to adjust the pH of the acidic
pinapple and states this gieves a further 100-150 mg/L of
organic nitrogen, this is considered by adding (NH4)3-citrate.
Several genera are sown by Malmgren on this medium, like
Dactylorhiza, Gymnadenia, Orchis and more. Likely because
of a typo Raennbaeck does not list vitamins. They are added
here as listed for all other Malmgren media in her paper.
Does not contain plant growth regulators. pH is adjusted to 5.7-5.9.
25.00 g per liter
Hygroscopic! Sealed in heavy duty plastic bag. To be
used immediately after opening.
Sterilization: autoclavable
Storage Temp.: RT (max. 1 year). For longer shelf life (>1 year) store at 4 ˇăC, for maximum
shelf life (>3 years) store at -20 ˇăC or below. Use up opened bags immediately and
do not store left-over powder from open bags!
Medium composition:
KH2PO4 0.0750 g/L
MgSO4 ˇ¤ 7H2O 0.0750 g/L
Ca3(PO4)2 0.0750 g/L
(NH4)3-citrate 0,6000 g/L
Malmgren vitamins (Prod. # L20090) 0.0366 g/L
Malmgren amino acids (Prod. # L20147) 0.3000 g/L
Pineapple powder, FD* (Prod. # L20127) 3.0000 g/L
Potato powder, FD** (Prod. # L20128) 5.0000 g/L
Activated charcoal (Prod. # L20161) 0.5000 g/L
Sucrose 10.3384 g/L
Hengduagar (Prod. # L20112) 5.0000 g/L
pH pre-adjusted with KOH
*equals 21 ml pineapple juice
** equals 25 g fresh potato

 

Close