L10125  Svante Malmgren Medium 1c (SMM 1c)

Complete instant medium with gelling agent, ready to use.
Contrary to his earlier notes, in 2006 Svante Malmgren stated that
amino acids would be suboptimal for cypripedium germination. He
revised his earlier medium accordingly, see media composition.
The medium was published in 2007 by L.M. Raennbaeck, Sweden.
Does not contain plant growth regulators. pH is adjusted to 5.7-5.9.
25 g per liter
Hygroscopic! Sealed in heavy duty plastic bag. To be
used immediately after opening.
Sterilization: autoclavable
Storage Temp.: RT (max. 1 year). For longer shelf life (>1 year) store at 4 ˇăC, for maximum
shelf life (>3 years) store at -20 ˇăC or below. Use up opened bags immediately and
do not store left-over powder from open bags!

Medium composition:
NH4NO3 0.1000 g/L
NH4H2PO4 0.1500 g/L
KH2PO4 0.0750 g/L
MgSO4 ˇ¤ 7H2O 0.0750 g/L
Ca3(PO4)2 0.0750 g/L
(NH4)3-citrate* 0.6000 g/L
Malmgren vitamins (Prod. # L20090) 0.0366 g/L
Pineapple powder, FD** (Prod. # L20127) 3.0000 g/L
Potato powder*** (Prod. # L20128) 5.0000 g/L
Activated charcoal (Prod. # L20161) 0.5000 g/L
Sucrose 10.8884 g/L
Hengduagar (Prod. # L20112) 4.5000 g/L
pH pre-adjusted with KOH
*Malmgren neutralizes the acidic pineapple
juice with NH3 in water, resulting in 100 to
150 mg additional nitrogen, substituted
here with (NH4)3-citrate.
**equals 21 ml pineapple juice
*** equals 25 g/L (21 cm3/L) fresh potato

 

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